Robust Acquisition of High Resolution Spatial Transcriptomes from Preserved Tissues with Immunofluorescence Based Laser Capture Microdissection
Xiaodan Zhang, Chuansheng Hu, Chen Huang, Ying Wei, Xiaowei Li, Miaomiao Hu, Hua Li, Ji Wu, Daniel Mark Czajkowsky, Yan Guo, Zhifeng Shao
This paper introduces a method to obtain high-resolution spatial transcriptomes from preserved tissues using immunofluorescence-based laser capture microdissection, demonstrating its effectiveness in analyzing transcript isoforms in mouse small intestine cells.
Frontiers in Cell and Developmental Biology · Mar 25, 2022Peer-reviewedGenomics