Amplification-Free Dual-Blocking Autocatalytic CRISPR-Cascade for Atto-Molar DNA Detection with Low Nonspecific Signal
Jongwon Lim, An Bao Van, Matthew Wester, Katherine Koprowski, Enrique Valera, Rashid Bashir
This paper introduces a dual-blocking CRISPR-Cascade system that reduces background signal and enables sensitive, amplification-free DNA detection.
Proceedings of the National Academy of Sciences · May 21, 2026Peer-reviewedMolecular Biology