Kinetic measurements of the fluorescent protein synthesis facilitate determination of the miRNA activity.
Philipp Malakhov, Yuzhe Wang, Wenyu Xue, Zain Nofal, Nikolay S. Ilyinsky, Anna V. Smirnova, Roman N. Chuprov‐Netochin, Margarita V. Pustovalova, Sergey V. Leonov, Julian Markovich Rozenberg
The paper develops miRNA sensors using fluorescent protein synthesis to measure miRNA activity, showing faster response times compared to degradation-based methods.
bioRxiv · Dec 19, 2025 · PreprintMolecular Biology