Design-Build-Test-Learn guided engineering of a whole-cell pyruvate biosensor based on transcription factor
Zihan Gao, Maria Suarez Diez, Pieter Candry
The paper develops a pyruvate biosensor in E. coli using Design-Build-Test-Learn cycles, achieving improved dynamic range and reduced leaky expression.
ACS Synthetic Biology · Feb 8, 2026Peer-reviewedSynthetic Biology