Magnetic Affinity-Based Purification of His-Tagged Proteins Using Ni2+-Functionalized Nanoparticles
Carolina Otonelo, Elisa de Sousa, Luciana C. Juncal, P. Mendoza Zélis, Sheila Ons, Claudia Rodríguez Torres, Carla Layana
This paper develops and validates a magnetic nanoparticle-based method for efficient purification of His6-tagged proteins, showing higher yield than traditional chromatography.
bioRxiv · Aug 4, 2025 · PreprintMolecular Biology