Molecular BiologybioRxivPreprint
Magnetic Affinity-Based Purification of His-Tagged Proteins Using Ni2+-Functionalized Nanoparticles
Carolina Otonelo, Elisa de Sousa, Luciana C. Juncal…·Carla Layana
This paper develops and validates a magnetic nanoparticle-based method for efficient purification of His6-tagged proteins, showing higher yield than traditional chromatography.
DOI 10.1101/2025.08.04.668490