Evolving a High-Performance Terminal Deoxynucleotidyl Transferase for Enzymatic DNA Synthesis
Stephanie M. Forget, Mikayla Krawczyk, Anders M. Knight, Charlene Ching, Rachelle A. Copeland, Niusha Mahmoodi, Melissa Mayo, James Nguyen, Amanda Tan, Mathew G. Miller, Jonathan A. Vroom, Stefan Lutz
This paper evolves a terminal deoxynucleotidyl transferase for enzymatic DNA synthesis using modified substrates, showing improved activity, template promiscuity, and thermostability.
Nucleic Acids Research · Feb 8, 2025Peer-reviewedBioengineering