A hybrid combination of in vitro cultured buccal mucosal cells using two different methodologies, complementing each other in successfully repairing a stricture-inflicted human male urethral epithelium
Akio Horiguchi, Toshihiro Kushibiki, Yoshine Mayumi, Masayuki Shinchi, Kenichiro Ojima, Yusuke Hirano, Shojiro Katoh, Masaru Iwasaki, Vaddi Surya Prakash, Koji Ichiyama, Rajappa Senthilkumar, Senthilkumar Preethy, Samuel J. K. Abraham
The paper describes a hybrid cell culture method combining 2D and 3D cultured cells from human buccal tissue to repair urethral stricture, showing increased epithelial markers in 3D cultures and higher IGF-1 secretion in 2D cultures.
Frontiers in Urology · Jan 14, 2026Peer-reviewedSynthetic Biology