Tetramethylrhodamine self-quenching is a probe of conformational change on the scale of 15 - 25 Angstroms
Paul Girvan, Liming Ying, Charlotte A. Dodson
The paper determines the distance-dependence of TMR self-quenching and bounds its dissociation timescale, validating it as an alternative to FRET for conformational change detection.
Chemical Communications · Jan 1, 2025Peer-reviewedBiophysics