Author

Alexei I. Slesarev

BioReliance (United States)ORCID 0000-0002-8672-7248Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Research Focus:variant calling· 2 Research Focus:benchmarking· 1 Assay:DNA methylation· 1 Organism:mouse· 1 Assay:whole-genome sequencing· 1

Frequent Co-Authors

A. KuziniD· 1 shared Audrey ChangiD· 1 shared Brendan Redler· 1 shared Colette A. CoteiD· 1 shared David E. Onions· 1 shared David S. RazafskyiD· 1 shared Jaya Onuska· 1 shared Katherine Achtien· 1 shared

Activity by Year

2019-2021 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 24 mutual co-author links

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1 shared paperGenomics

A. KuzinORCID

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2 papers

Recent·Most cited

RGEN-SEQ FOR HIGHLY SENSITIVE AMPLIFICATION-FREE SCREEN OF OFF-TARGET SITES OF GENE EDITORS

A. Kuzin, Brendan Redler, Jaya Onuska, Alexei I. Slesarev

The paper introduces RGEN-seq, a PCR-free next-generation sequencing method for detecting off-target sites of CRISPR-Cas9, showing improved sensitivity and workflow simplicity compared to existing methods.

Scientific Reports · Dec 8, 2021Peer-reviewedGenomics

CRISPR/Cas9 Targeted Capture Of Mammalian Genomic Regions For Characterization By NGS

Alexei I. Slesarev, Lakshmi Viswanathan, Yitao Tang, Trissa Borgschulte, Katherine Achtien, David S. Razafsky, David E. Onions, Audrey Chang, Colette A. Cote

The paper compares CRISPR/Cas9-based targeted capture methods with whole genome sequencing for detecting structural variants in CHO cell lines, finding that CRISPR/Cas9 reduces sequencing artifacts but lacks completeness for complex rearrangements.

Scientific Reports · Mar 5, 2019Peer-reviewedGenomics