CRISPR/Cas9 Targeted Capture Of Mammalian Genomic Regions For Characterization By NGS
Alexei I. Slesarev, Lakshmi Viswanathan, Yitao Tang, Trissa Borgschulte, Katherine Achtien, David S. Razafsky, David E. Onions, Audrey Chang, Colette A. Cote
The paper compares CRISPR/Cas9-based targeted capture methods with whole genome sequencing for detecting structural variants in CHO cell lines, finding that CRISPR/Cas9 reduces sequencing artifacts but lacks completeness for complex rearrangements.
Scientific Reports · Mar 5, 2019Peer-reviewedGenomics