A versatile high throughput strategy for cloning the env gene of HIV-1
Nitesh Mishra, Ayushman Dobhal, Shaifali Sharma, Kalpana Luthra
This paper describes a high-throughput method for cloning HIV-1 env genes using Phusion polymerase and overlap extension PCR, enabling efficient analysis of diverse env genes from infected individuals.
bioRxiv · Apr 1, 2020 · PreprintSynthetic Biology