Rocío del Carmen Bravo-Miana

This study identifies a critical technical limitation in SP-IRIS/IF platforms where non-vesicular artifacts persist after detergent treatment, highlighting the need for detergent lysis controls to ensure accurate single-EV phenotyping.

The paper proposes a workflow to analyze bacterial transcripts in RNAseq data from blood extracellular vesicles of people with Multiple Sclerosis to improve taxonomic profiling accuracy.