Molecular BiologyRNAPeer-reviewed
Nuclease-mediated depletion biases in ribosome footprint profiling libraries
Boris Zinshteyn, Jamie R Wangen, Boyang Hua, Rachel Green
The paper evaluates commercial RNA-seq depletion methods for ribosome footprint profiling and recommends custom biotinylated oligos over nuclease-based approaches due to their impact on data resolution.