Rachel Green

Oligo(uridine) tailing and exonucleolytic decay drive 40S ribosome degradation in response to stress

In starved HEK293T cells, RIOK3 recruits TUT7 and DIS3L2 to 40S ribosomes; TUT7 adds 3′ oligo(U) tails to 18S rRNA and DIS3L2 degrades it 3′ to 5′.

Substrate Binding Relieves Autoinhibition of the Mammalian GCN2 Kinase

This study shows that substrate binding, not tRNA or ribosome interactions, activates GCN2 kinase by relieving autoinhibition and promoting autophosphorylation.

Conformational diversity of collided ribosomes determines stress signaling

The paper shows that different drugs inducing ribosome collisions lead to distinct cellular responses through varied signaling pathways and structural changes in ribosomes.

A novel RRM activity orchestrates ribosome homeostasis

40S subunit binding to a previously unrecognised RRM in LARP1 activates its TOP-binding HEAT repeats, licensing repression of ribosomal protein mRNA translation in human cells.

SERBP1 is a master regulator of ribosome interactions

This study identifies SERBP1 as a regulator of ribosome interactions, showing its role in modulating factors involved in mRNA degradation, translational control, and ribosome quality control.

A conserved dimerization element is required for protein kinase activation by trans-autophosphorylation

This study identifies a conserved helix (G) mediating dimerization during trans-autophosphorylation in kinases across species, showing it is required for activation despite structural diversity.

The RNA helicase HrpA rescues collided ribosomes in E. coli

The paper shows that HrpA, an RNA helicase in E. coli, rescues stalled ribosomes by splitting collided ribosomes into subunits using ATP hydrolysis.

B. subtilis MutS2 splits stalled ribosomesinto subunits without mRNA cleavage

This study shows that MutS2 in B. subtilis splits stalled ribosomes into subunits without cleaving mRNA, differing from the E. coli SmrB mechanism.

LARP1 senses free ribosomes to coordinate supply and demand of ribosomal proteins

This study shows LARP1 binds free ribosomal subunits to repress TOP mRNAs, coordinating ribosome protein synthesis with cellular demand.

Bursting Translation on Single mRNAs in Live Cells

The paper tracks single mRNA translation in live cells, revealing that translation occurs in bursts and is influenced by 5' UTR structure and environmental signals.

Distinct ribosome states trigger diverse mRNA quality control pathways

The paper investigates mRNA quality control pathways in yeast, showing that Syh1 is central to no-go decay (NGD) while Not5 has a minor role, and ribosome profiling reveals distinct ribosomal states for NGD and COMD.

Ribosome collisions in bacteria promote ribosome rescue by triggering mRNA cleavage by SmrB

The paper identifies SmrB as a factor that cleaves mRNAs upstream of stalled ribosomes in bacteria, promoting ribosome rescue through ribosome collisions.

Genetic screens identify connections between ribosome recycling and nonsense mediated decay

The paper identifies factors linking ribosome recycling and nonsense mediated decay in yeast using genetic screens and reporter constructs.

GIGYF2 and 4EHP Inhibit Translation Initiation of Defective Messenger RNAs to Assist Ribosome-Associated Quality Control

The paper identifies GIGYF2 and 4EHP as translation inhibitors that suppress re-initiation of defective mRNAs, aiding ribosome-associated quality control and preventing toxic protein accumulation.

eEF3 promotes late stages of tRNA translocation on the ribosome

This study identifies eEF3's role in promoting tRNA dissociation from the E site during translation elongation in fungi, revealing structural insights into the yeast translation cycle.

Nuclease-mediated depletion biases in ribosome footprint profiling libraries

The paper evaluates commercial RNA-seq depletion methods for ribosome footprint profiling and recommends custom biotinylated oligos over nuclease-based approaches due to their impact on data resolution.

Stop Codon Context Influences Genome-Wide Stimulation of Termination Codon Readthrough by Aminoglycosides

The paper investigates how stop codon context affects genome-wide stop codon readthrough induced by aminoglycosides, showing that both premature and normal termination codons are affected.

The endonuclease Cue2 cleaves mRNAs at stalled ribosomes during No Go Decay.

The paper identifies Cue2 as the endonuclease responsible for cleaving mRNAs at stalled ribosomes during No Go Decay and shows it acts as a secondary decay pathway.

Precision genome editing using synthesis-dependent repair of Cas9-induced DNA breaks

This paper describes a method for efficient precision genome editing using linear donor DNAs with ~35bp homology arms for synthesis-dependent strand annealing repair of Cas9-induced breaks.