Accurate detection of metagenomic strain-level associations using average nucleotide identity with StrainSpy
Sudaraka Mallawaarachchi, Kshitij Tandon, Nikhil Rajan, Vanessa R. Marcelino, Shahneen Kaur Sandhu, Sammy Bedoui, Danielle J. Ingle, Ashray Gunjur, Gerry Q. Tonkin‐Hill
StrainSpy fits zero-inflated beta mixed models to containment ANI, reaching >98% simulated sensitivity versus ~79% for logistic regression and detecting colorectal-cancer E. coli strain shifts.
bioRxiv · Oct 3, 2026 · PreprintMicrobiology✦ In-depth digest