SARS-CoV-2 genomes recovered by long amplicon tiling multiplex approach using nanopore sequencing and applicable to other sequencing platforms
Paola Cristina Resende, Fernando Couto Motta, Sunando Roy, Luciana Appolinario, Allison de Araújo Fabri, Joilson Xavier, Kathryn Harris, Aline da Rocha Matos, Bráulia Costa Caetano, Maria Ogrzewalska, Milene Dias Miranda, Cristiana Couto Garcia, André Abreu, Rachel J. Williams, Judith Breuer, Marilda Mendonça Siqueira
Three multiplex tiling protocols using a single primer set recover long amplicons of SARS-CoV-2 directly from clinical sample total RNA for sequencing on Nanopore, Illumina, or Sanger platforms.
bioRxiv · Apr 30, 2020 · PreprintMolecular Biology