The bacterial hitchhiker's guide to COI: Universal primer-based COI capture probes fail to exclude bacterial DNA, but 16S capture leaves metazoa behind
Sanni Hintikka, Jeanette E. L. Carlsson, Jens Carlsson
This study evaluates the use of universal primers as capture probes to isolate or remove DNA from eDNA samples, finding that COI capture probes fail to exclude bacterial DNA while 16S capture misses metazoans.
Metabarcoding and Metagenomics · May 31, 2022Peer-reviewedMolecular Biology