Stable isotope tracer captures the anabolic response of human skeletal muscle microtissues undetected by puromycin labeling
Yekaterina Tiper, Cassidy T. Tinline‐Goodfellow, Penney M. Gilbert, Daniel R. Moore
This study compares stable isotope tracers and puromycin labeling in measuring muscle protein synthesis in engineered skeletal muscle, finding tracers detect anabolic responses missed by puromycin.
bioRxiv · Sep 12, 2025 · PreprintBioengineering