Filomena Freitas

Polysaccharides in cryopreservation: multidimensional systematic review of extremophilic traits and the role of selective pressure in structure-function relationships

This paper reviews extremophilic polysaccharides' structure-function relationships in cryopreservation, identifying key traits for cold adaptation and their potential in designing non-toxic cryoprotectants.

Tosylation-mediated uronic acid knockout from cryoprotective FucoPol revealed the importance of polyanionicity in ice growth disruption

This study shows that removing uronic acids from FucoPol reduces its cryoprotective activity, highlighting the importance of polyanionicity in ice growth disruption.

Elucidation of the polysaccharide cryoprotection mechanism: kinetic inhibition, thermodynamic Gibbs-Thomson modulation and volumetric confinement

This paper explains how gel-forming polysaccharides protect cells from freezing damage through kinetic inhibition, Gibbs-Thomson modulation, and volumetric confinement mechanisms.