Author

João Carlos Lima

Universidade Nova de LisboaORCID 0000-0003-0528-1967Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Biochemistry· 1 Biophysics· 1 Molecular Biology· 1

Signature Assays, Organisms & Methods

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Approach:statistical model· 2 Approach:benchmark study· 1 Research Focus:database / resource· 1 Approach:experimental assay· 1

Frequent Co-Authors

Bruno M. GuerreiroiD· 3 shared Filomena FreitasiD· 3 shared Jorge Alexandre Monteiro de Carvalho SilvaiD· 3 shared

Activity by Year

2025-2025 · peak 3/yr

Co-Author Collaboration Network

3 top collaborators · 3 mutual co-author links

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3 shared papersMolecular Biology

Bruno M. GuerreiroORCID

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3 papers

Recent·Most cited

Molecular BiologyCarbohydrate PolymersPeer-reviewedSep 29, 2025

Polysaccharides in cryopreservation: multidimensional systematic review of extremophilic traits and the role of selective pressure in structure-function relationships

Bruno M. Guerreiro, João Carlos Lima, Jorge Alexandre Monteiro de Carvalho Silva, Filomena Freitas

This paper reviews extremophilic polysaccharides' structure-function relationships in cryopreservation, identifying key traits for cold adaptation and their potential in designing non-toxic cryoprotectants.

6 citations

statistical modelbenchmark study

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BiochemistrybioRxivPreprintAug 27, 2025

Tosylation-mediated uronic acid knockout from cryoprotective FucoPol revealed the importance of polyanionicity in ice growth disruption

Bruno M. Guerreiro, João Carlos Lima, Jorge Alexandre Monteiro de Carvalho Silva, Filomena Freitas

This study shows that removing uronic acids from FucoPol reduces its cryoprotective activity, highlighting the importance of polyanionicity in ice growth disruption.

1 citation

experimental assay

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BiophysicsbioRxivPreprintAug 27, 2025

Elucidation of the polysaccharide cryoprotection mechanism: kinetic inhibition, thermodynamic Gibbs-Thomson modulation and volumetric confinement

Bruno M. Guerreiro, João Carlos Lima, Jorge Alexandre Monteiro de Carvalho Silva, Filomena Freitas

This paper explains how gel-forming polysaccharides protect cells from freezing damage through kinetic inhibition, Gibbs-Thomson modulation, and volumetric confinement mechanisms.

DOI 10.1101/2025.08.27.672675

statistical model

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