Correcting errors in PCR-derived libraries for rare allele detection by reconstructing parental and daughter strand information
Hyeonseob Lim, Soyeong Jun, Taehoon Kim, Ji Hyun Lee, Duhee Bang
This paper introduces SPIDER-seq, a method to detect rare alleles in PCR-derived libraries by tracking molecular identity through overwritten barcodes, achieving sensitivity down to 0.125% mutation frequency.
Communications Biology · Jul 24, 2025Peer-reviewedBioinformatics