BioinformaticsCommunications BiologyPeer-reviewed
Correcting errors in PCR-derived libraries for rare allele detection by reconstructing parental and daughter strand information
Hyeonseob Lim, Soyeong Jun, Taehoon Kim…·Duhee Bang
This paper introduces SPIDER-seq, a method to detect rare alleles in PCR-derived libraries by tracking molecular identity through overwritten barcodes, achieving sensitivity down to 0.125% mutation frequency.