Duhee Bang

Correcting errors in PCR-derived libraries for rare allele detection by reconstructing parental and daughter strand information

This paper introduces SPIDER-seq, a method to detect rare alleles in PCR-derived libraries by tracking molecular identity through overwritten barcodes, achieving sensitivity down to 0.125% mutation frequency.

Multiplexed single-cell RNA-seq via transient barcoding for drug screening

The paper develops a transient barcoding method for multiplexed scRNA-seq to analyze multiple drug treatments in a single experiment, revealing unique transcriptome responses and target-specific gene signatures.

Lineage tracing using a Cas9-deaminase barcoding system targeting endogenous L1 elements

This paper develops a genetic barcode system using a Cas9-deaminase to trace cell lineage in mammals, showing accurate reconstruction of lineage from bulk and single-cell data.

TnClone: high-throughput clonal analysis usingTn5-mediated library construction and de novo assembly

TnClone reduces cloning validation costs by 20-fold using NGS and detects low-frequency mutants with 99.4% sensitivity.