Pasquale Memmolo

Label-Free Nucleoli Measurement by 3D Holo-Tomographic Flow Cytometry Using Biolens Phase Compensation

This paper introduces Biolens Phase Compensation to enable 3D nucleoli measurement in HTFC, achieving intranuclear specificity comparable to fluorescence microscopy.

Impact of Image Representation on Deep Learning-Based Single-Cell Classification by Holographic Imaging Flow Cytometry

This paper evaluates image representations in holographic imaging flow cytometry to balance classification accuracy and computational efficiency for deep learning-based cell type classification.

Establishing label-free quantitative X-ray dose-response profiling by Holo-Tomographic Flow Cytometry

This paper introduces HTFC, a label-free flow cytometry method for X-ray dose-response profiling, showing high correlation with clonogenic assays and faster results.

Profiling co-occurrent morphological phenotypes and their degree of expression severity in vacuolated cells by holo-tomographic flow cytometry and fractal analysis

The paper introduces a pipeline to profile co-occurring morphological phenotypes in vacuolated cells using HTFC and fractal analysis, assigning severity levels to each phenotype.

Holotomography-driven learning for in-silico staining of single cells in flow cytometry avoiding co-registration

This paper introduces a Holotomography-driven learning method for virtual staining in flow cytometry without requiring co-registration, enabling label-free single-cell analysis.

Self-consistent automatic retrieval of single cell rotation enables highly reliable holo-tomographic flow cytometry

This paper introduces a self-consistent method for automatically retrieving single cell rotations in holo-tomographic flow cytometry, achieving full automation and superior accuracy.

Quantitative profiling of lysosomal accumulation through label-free biomarkers via High-Content Holo-Tomographic Flow Cytometry

This paper introduces HTFC to quantify lysosomal accumulation in LSDs, identifies novel biomarkers, and links 3D lysosomal structure to therapeutic efficacy.

Simulating 3D refractive index distributions of suspended cells

This paper introduces a method to simulate 3D refractive index tomograms of suspended cells and provides a benchmark dataset for algorithm development.