Diego Luis Medina

Lysosomal proteome and lipidome analyses of intestinal cells reveal the crucial role of bis(monoacylglycero)phosphate for autophagosome-lysosome fusion

This study identifies BMP's role in autophagosome-lysosome fusion by analyzing lysosomal proteomes and lipidomes in GNPTAB-deficient intestinal cells.

Label-Free Nucleoli Measurement by 3D Holo-Tomographic Flow Cytometry Using Biolens Phase Compensation

This paper introduces Biolens Phase Compensation to enable 3D nucleoli measurement in HTFC, achieving intranuclear specificity comparable to fluorescence microscopy.

Ion Mobility-Guided Tandem Mass Spectrometry Imaging Resolves Bis(monoacylglycero)phosphate and Phosphatidylglycerol Isomers in Tissue

This paper develops an ion mobility-guided MSI workflow to distinguish BMP and PG isomers in tissue, showing reduced cross-talk and enabling structural assignment in mouse brain and a CLN3-knockout model.

Holotomography-driven learning for in-silico staining of single cells in flow cytometry avoiding co-registration

This paper introduces a Holotomography-driven learning method for virtual staining in flow cytometry without requiring co-registration, enabling label-free single-cell analysis.

Quantitative profiling of lysosomal accumulation through label-free biomarkers via High-Content Holo-Tomographic Flow Cytometry

This paper introduces HTFC to quantify lysosomal accumulation in LSDs, identifies novel biomarkers, and links 3D lysosomal structure to therapeutic efficacy.

PAK6 promotes neuronal autophagy by regulating TFEB nuclear translocation.

This study identifies PAK6 as a positive regulator of neuronal autophagy through TFEB nuclear translocation, with implications for neurodegenerative disease treatment.

Mitochondrial collapse links PFKFB3-promoted glycolysis with CLN7/MFSD8 neuronal ceroid lipofuscinosis pathogenesis

This study identifies a metabolic shift driven by PFKFB3 in CLN7 disease, showing that targeting glycolysis can alleviate disease hallmarks in a mouse model and patient-derived cells.

gene2drug: a Computational Tool for Pathway-based Rational Drug Repositioning

gene2drug prioritizes drugs based on their impact on transcriptional pathways of target genes, validated both computationally and experimentally for two drug repositioning cases.

Integrated Structure-Transcription analysis of small molecules reveals widespread noise in drug-induced transcriptional responses and a transcriptional signature for drug-induced phospholipidosis

The paper integrates drug chemical structures with transcriptional responses to identify noisy responses and a transcriptional signature for phospholipidosis.