Enhancing Proteoform Sequence Coverage Using Top-Down Mass Spectrometry with In-Source Fragmentation and Middle-Down Mass Spectrometry
Xingzhao Xiong, Letu Qingge, Binhai Zhu, Xiaowen Liu
This study evaluates top-down and middle-down mass spectrometry approaches to enhance proteoform sequence coverage using in-source fragmentation and partial digestion, achieving over 90% coverage for three proteins.
Analytical Chemistry · Feb 2, 2026Peer-reviewedBioinformatics