Author

Sebastian Gude

Novo Nordisk FoundationORCID 0000-0003-0688-5025Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Microbiology· 1

Signature Assays, Organisms & Methods

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Assay:CRISPR screen· 1 Organism:E. coli· 1 Approach:experimental assay· 1 Research Focus:genome assembly· 1

Frequent Co-Authors

Andreas Birk BertelseniD· 1 shared Morten H. H. NørholmiD· 1 shared

Activity by Year

2025-2025 · peak 1/yr

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1 shared paperMicrobiology

Andreas Birk BertelsenORCID

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1 paper

Recent·Most cited

MicrobiologybioRxivPreprintJun 19, 2025

Iterative in vivo cut'n'paste of chromosomal loci in Escherichia coli K-12 using synthetic DNA

Sebastian Gude, Andreas Birk Bertelsen, Morten H. H. Nørholm

This paper introduces an in vivo method to iteratively relocate chromosomal loci in E. coli using synthetic DNA, enabling scarless genome editing without requiring external DNA manipulation.

DOI 10.1101/2025.06.19.660532

E. coliCRISPR screen

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