Molecular BiologyScientific ReportsPeer-reviewed
Removing auto-activators from yeast-two-hybrid assays by conditional negative selection
Devendra Shivhare, Magdalena Musialak‐Lange, Irene Julca…·Marek Mutwil
The paper develops a negative selection method to remove auto-activators from yeast-two-hybrid assays using pGAL2-URA3 and 5-FOA treatment, reducing false-positive hits.
16 citations