Nicolas T. Wirth

This study improves acetate tolerance in Pseudomonas putida through adaptive laboratory evolution and identifies gacA and fabB as key targets for efficient acetate assimilation.

This paper engineers a synthetic C2 auxotrophy in Pseudomonas putida to enable alternative sugar catabolism via the phosphoketolase shunt, improving growth through metabolic rewiring.

The paper parameterizes five bacterial gene expression systems using a standardized vector format to compare their performance across different inducers.