Molecular BiologybioRxivPreprint
Molecular principles of CRISPR-Cas13 mismatch intolerance enable selective silencing of point-mutated oncogenic RNA with single-base precision
Carolyn Shembrey, Ray Yang, Joshua M. L. Casan…·Mohamed Fareh
This paper develops CRISPR-Cas13 crRNAs that selectively silence oncogenic SNV transcripts with single-base precision by exploiting mismatch intolerance in target-spacer interactions.
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