Design of an effective sgRNA for CRISPR/Cas9 knock-ins in polyploid Synechocystis sp. PCC 6803
María Isabel Nares-Rodriguez, Esther Karunakaran
The paper develops an effective sgRNA for CRISPR/Cas9-mediated knock-ins in polyploid Synechocystis sp. PCC 6803, enabling reliable genetic modification.