Synthetic BiologyACS Synthetic BiologyPeer-reviewed
Development of approaches and identifying limitations towards functional yeast surface display of FLS2
Benedikt Dolgikh, Samantha Schulte, Daniel Woldring
The paper develops methods to improve yeast surface display of FLS2 for detecting bacterial ligands, identifies glycosylation as a limiting factor, and shows how modifying glycosylation sites enhances binding specificity.
DOI 10.1021/acssynbio.5c00884