Experimental evolution of gene essentiality in bacteria
Liang Bao, Zan Zhu, Ahmed Ismail, Bin Zhu, Vysakh Anandan, Marvin Whiteley, Todd O. Kitten, Ping Xu
The paper generates essential gene deletion mutants in Streptococcus sanguinis and identifies suppressor mutations through experimental evolution, revealing new gene and pathway relationships.
mBio · Oct 31, 2025Peer-reviewedEvolutionary Biology