Jara Ballestin Ballestin

Int&in is a web server that predicts active and inactive split sites in inteins using a Gaussian Naive Bayes model with 0.76 accuracy.

The paper identifies new split sites in Npu DnaE and gp41-1 inteins, enabling functional three-piece versions for biotechnological applications.

The paper expands the SiMPl plasmid toolbox to include spectinomycin/streptomycin resistance by introducing a new plasmid pair and validating a strategy for splitting antibiotic resistance enzymes.