Author

Valeria Dountcheva

University of Massachusetts BostonORCID 0009-0004-4486-2515Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Developmental Biology· 1 Molecular Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Assay:imaging· 1

Frequent Co-Authors

Labib RouhanaiD· 2 shared Athanasios BubulyaiD· 1 shared Vaidehi Patel· 1 shared

Activity by Year

2025-2026 · peak 1/yr

Co-Author Collaboration Network

3 top collaborators · 2 mutual co-author links

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2 shared papersMolecular Biology

Labib RouhanaORCID

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2 papers

Recent·Most cited

Molecular BiologymicroPublicationPeer-reviewedFeb 11, 2026

Directionality bias in T/A cloning

Valeria Dountcheva, Athanasios Bubulya, Labib Rouhana

The paper identifies a directional bias in T/A cloning with the pGEM-T Vector System, showing over 74% of inserts are in the 'sense' direction relative to the T7 promoter.

DOI 10.17912/micropub.biology.002065

experimental assay

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Developmental BiologybioRxivPreprintNov 10, 2025

Acridine Orange dye for long-term staining and live imaging of cnidarian development and regeneration

Vaidehi Patel, Valeria Dountcheva, Labib Rouhana

This study evaluates Acridine Orange as a staining tool for long-term live imaging of cnidarian development and regeneration, finding it suitable for Nematostella vectensis but not for cell tracking via microinjection.

DOI 10.1101/2025.11.10.687709

imagingexperimental assay

𝕏 PostBluesky