Tessa M. Firestone

The paper provides a detailed CUT&RUN protocol for high-resolution epigenomic profiling, including best practices and controls for efficient target profiling.

The paper investigates how nucleosome context influences chromatin reader binding preferences using biochemical and genomic approaches, revealing that multivalent engagement often has regulatory effects beyond simple additivity.

The paper shows that the PBAF complex in melanoma facilitates REST occupancy at repressive chromatin, leading to upregulation of synaptic transcripts when ARID2 is mutated.