GenomicsRoyal Society Open SciencePeer-reviewed
Cas9-based enrichment for targeted long-read metabarcoding
Lucia Nikolaeva-Reynolds, Christopher Cammies, Rosemary Crichton, Thomas E. Gorochowski
This paper introduces a Cas9-based method for targeted long-read metabarcoding, enabling multiplexed enrichment of marker genes without PCR, and demonstrates its effectiveness in characterizing complex microbial and nematode communities.