Inhibitory inputs to avian ITD circuits

Abstract

In birds, detection of sound source azimuth begins in the nucleus laminaris (NL), which computes interaural time differences (ITDs). Inhibition has been proposed to protect NL neurons from losing ITD sensitivity with increasing sound level, although the nature of this inhibition is incompletely understood. Anatomical studies show GABAergic synapses throughout NL and the first order nuclei, while in vitro studies in chicken NL showed that depolarizing, GABAergic inhibitory postsynaptic potentials (IPSPs) shorten membrane time constants, perhaps to allow the membrane potential to follow rapid synaptic currents accurately over a range of sound levels. Given the importance of inhibition in regulating auditory brainstem activity, we examined the nature of the inhibitory input to NL in vivo. The Superior Olivary Nucleus (SON) is the major source of descending GABAergic inhibition to the ipsilateral nucleus magnocellularis (NM), nucleus angularis (NA) and NL, and receives excitatory input from NA and NL. We used viral tracers to reveal projections from SON to NL, and characterized response types within SON. In NL, we isolated inhibitory synaptic contributions from extracellular field potentials through the iontophoretic application of blockers of GABA (gabazine) or glycine (strychnine). These blockers increase the onset and offset responses evoked by tonal stimuli, but did not shift best ITD. The co-application of gabazine and strychnine revealed supra-linear summation of GABA and glycine. Profiles of synaptic activation revealed more prominent inhibition following stimulus offset, suggesting inputs to NL originate from both the sustained and offset response types of the SON. These heterogeneous responses may represent separate SON neuronal populations.