Light-dependent switching of circling handedness in microswimmer navigation

Abstract

Many swimming microorganisms navigate their environment by modulating the curvature of their swimming trajectories in response to external cues. Here, we show that the biflagellate alga Chlamy-domonas reinhardtiiswims in circles and actively switches its trajectory handedness in response to orthogonal illumination: the cell swims counterclockwise at low light intensities yet clockwise at high light intensities. This handedness switching arises from light-dependent modulation of flag-ellar beating, including changes in beat extension, phase, and--crucially--beat plane orientation. Using high-speed imaging and hydrodynamic modeling, we reveal that this beat plane reorientation is critical for Chlamydomonas to swim orthogonally to light as well as to dynamically modulate its trajectory curvature, enabling transitions between global exploration and localized searching in spatially structured light fields. Our results establish beat plane reorientation as a novel mechanism for curvature control in microswimmer navigation.