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Molecular BiologybioRxiv
Preprint

Simple, rapid, and sensitive assay for the quantification of total polysaccharides to estimate extracellular polymeric substances (EPS) in soil

Glade D. BogariD, Jay T. LennoniD, Holly Vander SteliD, Sarah E. EvansiD

Abstract

Current methods for quantifying total polysaccharides in soil are expensive or require prolonged incubation of hazardous reagents at 100{degrees}C in glass vessels. We present a fast, sensitive, and inexpensive spectrophotometric assay quantifying polysaccharides in aqueous solution, featuring an instant reaction of room-temperature reagents using disposable tubes and cuvettes.

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