Gene cloning and construction of prokaryotic and plant expression vectors of RICIN-A-Chain/PAP-S1 fusion protein and its inhibition of protein synthesis

Abstract

INTRODUCTION INTRODUCTION MATERIALS AND METHODS Design of fusion DNA... DNA Sequencing RESULTS AND DISCUSSION Conclusion References Plants produce toxic proteins known as ribosome inactivating proteins (RIPs) essential to their defense mechanism against outside pathogens. RIPs are RNA N-glycosidases that function by irreversibly inhibiting protein synthesis through the removal of one or more adenine residues from ribosomal RNA (rRNA) [1]. In addition, certain RIPs can remove adenine from DNA and other polynucleotides for which reason they are also known as polynucleotide adenosine glycosidases. Pokeweed antiviral protein (PAP), a RIP from Phytolacca americana, can cleave not only adenine, but also guanine from the rRNA of Escherichia coli. RIPs are usually categ ...