biodegradable polymer synthesis and properties

Characterisation of PHA synthase (PhaC) from the thermophilic bacterium Caldimonas thermodepolymerans DSM15344

This study characterizes PHA synthase (PhaC) from Caldimonas thermodepolymerans, determining its optimal conditions and structural features.

Bioplastic Production from simulated 100% in situ Mars resources

This study demonstrates the production of PHA bioplastic from simulated 100% Martian resources using engineered microbes.

Robust cellular transformations of PET deconstruction products by import of glycol esters

This study demonstrates that E. coli can efficiently uptake and convert PET deconstruction products into upcycled reduction products with high yields.

Engineering Pseudomonas putida KT2440 for open-loop upcycling of mixed plastics

This study engineers Pseudomonas putida KT2440 to co-utilize five plastic monomers from mixed plastics, achieving complete substrate depletion and producing (R)-3-hydroxybutyrate.

Engineering xylose metabolism for diverse polyhydroxyalkanoates synthesis in Halomonas TD

This paper engineers Halomonas TD to produce diverse polyhydroxyalkanoates from xylose and glucose, achieving high yields of PHB and terpolymers through pathway modifications.

Material Composition and Implantation Site Affect in vivo Device Degradation Rate

This study investigates how material composition and implantation site affect in vivo degradation rates of tissue engineering devices using CT-visible radiopaque composites.

Tailoring Precise Genomic Integration Toward Isolate-to-Industry Strain Development for Scalable High-Titer Production of Polyhydroxyalkanoate

The paper develops genomic integration tools to engineer a halophilic strain for high-titer PHA production, achieving scalable and cost-effective industrial fermentation.

Salt-induced osmotic stress remodels osmoadaptive gene expression and physiology in the polyhydroxyalkanoate-accumulating thermophilic bacterium Caldimonas thermodepolymerans

This study examines how osmotic stress affects gene expression and physiology in Caldimonas thermodepolymerans, showing increased PHA accumulation and changes in osmoprotection and metabolism.

PETase Kubu enables near-complete enzymatic depolymerization of commercial PLA/PBAT blend mulch film

This paper demonstrates that Kubu, a PETase from Kutzneria buriramensis, efficiently degrades commercial PLA/PBAT mulch films, achieving 95% mass loss in 96 hours and releasing monomers detected by LC-MS/MS and HPLC.

Spore-Based Biocomposite Thermoplastic Polyesters with Enhanced Toughness and Programmable Disintegration

This paper develops spore-based biocomposite thermoplastic polyesters with enhanced toughness and programmable disintegration through integration of heat-shock-tolerized Bacillus subtilis spores.

Integrated valorization of glycerol and PET into lipids and PHAs using an engineered Yarrowia lipolytica strain and a Pseudomonas-Comamonas consortium

This paper presents a yeast-bacterial workflow to convert glycerol and PET into lipids and bioplastics using engineered strains and a consortium.

PhaB-independent poly(3-hydroxybutyrate) production in the thermophilic hydrogen-oxidizing bacterium Hydrogenophilus thermoluteolus

This study shows that Hydrogenophilus thermoluteolus can produce PHB without PhaB enzymes, suggesting alternative metabolic pathways for PHB synthesis.

Burkholderia vietnamiensis Genes Involved in Extracellular Medium-Chain-Length Polyhydroxyalkanoate Degradation

This paper identifies genes in Burkholderia vietnamiensis responsible for extracellular degradation of medium-chain-length polyhydroxyalkanoates using transposon mutagenesis, Tn-seq, and CAST.

Biochemical Upcycling of PET via Glycolysis and Engineered Microbial Consortia

This paper presents a biochemical approach using glycolysis and engineered microbial consortia to upcycle post-consumer PET into PHAs, demonstrating efficient depolymerization and monomer conversion.

New-to-nature PHA synthase design using deep learning

This study uses a conditional variational autoencoder to design new-to-nature PHA synthases, which are validated for activity in yeast and show potential for expanding PHA material properties.

A Toolbox for Biomanufacturing of Functionalised PHA Nanoparticles with C. necator

The paper develops a platform for producing functionalised PHA nanoparticles using C. necator, demonstrating customisable material properties and bioprocess improvements.

Identification and characterization of a poly(ϵ-caprolactone)-degrading enzyme with a unique sequence profile from the marine bacterium Alloalcanivorax gelatiniphagus

The paper identifies and characterizes a PCL-degrading enzyme from Alloalcanivorax gelatiniphagus, showing substrate specificity differences from known PET hydrolases.

Design and Synthesis of Peptide-Polyester Conjugates for Cell-Mediated Scaffold Degradation

This paper designs and synthesizes peptide-polyester conjugates that degrade in response to cell-mediated protease activity, enhancing tissue regeneration.

Systematic development of degradable polyester biomaterials via ring-opening copolymerization of succinic anhydride and epoxides

This study develops degradable polyester biomaterials via ROCOP, showing that monomer selection significantly affects degradation behavior and material properties.

Insights into transcriptional expression and putative functions of multiple polyhydroxyalkanoate synthase paralogs in Haloferax mediterranei

This study investigates the transcriptional expression and potential functions of three phaC paralogs in Haloferax mediterranei under different physiological conditions, revealing their active expression and growth-phase-dependent regulation.

Cultivation in a Natural Microbial Community Enhances the Industrial Performance of a Genetically Engineered Cyanobacterium for Bioplastic Production

This paper shows that integrating a genetically engineered cyanobacterium into a natural microbial community improves its resilience and PHB production, making it suitable for industrial bioplastic production.

Improved nylon polymerization using amide diads

This paper demonstrates a method to produce higher-molecular-weight nylons using chemically-synthesized diads and biosynthetic amide synthetases, expanding the range of usable precursors.

The redefined role of PhaG and CoA ligases in medium-chain-length 3-hydroxy acid and polyhydroxyalkanoate production in Pseudomonas putida

This paper redefines the role of PhaG and CoA ligases in PHA biosynthesis in Pseudomonas putida and demonstrates engineered production of 3-hydroxyacids.

Enrichment of bioplastic degraders in mesophilic compost from widespread degradation potential in the environment

The paper investigates PLA hydrolytic activity in various environments and a trained compost, finding that the compost selects for more efficient degraders despite similar detection frequencies.

Bioplastic Production Potential of Azospira suillum PS: Growth-Associated PHB Production Under Aerobic and Anaerobic Conditions

This study demonstrates Azospira suillum PS can produce PHB under both aerobic and anaerobic conditions with perchlorate as an electron acceptor, highlighting its potential for sustainable bioplastic production.

A systems-level insight into PHB-driven metabolic adaptation orchestrated by the PHB-binding transcriptional regulator AniA (PhaR)

This study identifies PhaR as a key regulator of PHB-mediated metabolic adaptation in S. meliloti, revealing its impact on carbon metabolism and exopolysaccharide production.

Mesophilic compostability of polylactic acid and the associated microbiome as revealed by metagenomics

This study shows mesophilic composts can degrade polylactic acid (PLA) through a microbial community with hydro-lyase enzymes, overcoming the need for thermophilic temperatures.

Establishing Vibrio natriegens as a high-performance host for acetate-based poly-3-hydroxybutyrate production

This study enhances Vibrio natriegens' ability to produce poly-3-hydroxybutyrate from acetate through adaptive evolution and metabolic engineering, achieving high productivity and biomass accumulation.

Synergistic Action of Commercial Lipase, Lipoxygenases and Proteinase towards PLA Depolymerization

This study shows that combining Proteinase K with lipase and lipoxygenase significantly enhances PLA degradation, with lipase showing the strongest synergistic effect.

Tracking polyhydroxyalkanoate biosynthesis in thermophilic microorganisms

This study uses bioinformatics to screen thermophilic genomes for polyhydroxyalkanoate synthesis genes, finding them rare in extreme thermophiles but present in some archaea.