Post-Assay Photogelation Enables Flow-Cytometric Sorting and Recovery of Bacterial Cocultures from Microfluidic Droplets

High-throughput droplet microfluidics can compartmentalize bacterial interactions, but recovering droplets displaying phenotypes of interest often requires custom fluorescence-activated droplet-sorting instrumentation. Here, we introduce post-assay photogelation to decouple the material requirements of bacterial coculture from those of commercial flow sorting. Bacteria are cocultured in initially aqueous water-in-oil droplets containing photoreactive polymer precursors. After interaction phenotypes develop, ultraviolet exposure converts the droplets into mechanically stable hydrogel particles that can be transferred to an aqueous carrier and sorted using a commercial benchtop cell sorter. The sorted particles can subsequently be degraded enzymatically to release the encapsulated bacteria. We show that the timing of gelation alters bacterial growth and spatial distribution within droplets, with post-assay gelation supporting greater and more uniformly distributed growth than culture in preformed hydrogels. Using two fluorescent bead-encoded hydrogel-particle populations, we demonstrate sorting to greater than 99% purity. As an end-to-end demonstration, we cocultured sfGFP-expressing Escherichia coli Nissle 1917 with a cultured human nasal bacterial community and found that E. coli Nissle became the predominant detectable population under the tested conditions with possible inhibition of the cultured nasal bacteriome. This liquid-to-solid transition provides an accessible interface between aqueous bacterial droplet assays, commercial particle sorting, and downstream microbial analysis.