Topology independent structural matching discovers novel templates for protein interfaces

Abstract

MotivationProtein-protein interactions (PPI) are essential for the function of the cellular machinery. The rapid growth of protein-protein complexes with known 3D structures offers a unique opportunity to study PPI to gain crucial insights into protein function and the causes of many diseases. In particular, it would be extremely useful to compare interaction surfaces of monomers, as this would enable the pinpointing of potential interaction surfaces based solely on the monomer structure, without the need to predict the complete complex structure. While there are many structural alignment algorithms for individual proteins, very few have been developed for protein interfaces, and none that can align only the interface residues to other interfaces or surfaces of interacting monomer subunits in a topology independent (non-sequential) manner.\n\nResultsWe present InterComp, a method for topology and sequence-order independent structural comparisons. The method is general and can be applied to various structural comparison applications. By representing residues as independent points in space rather than as a sequence of residues, InterComp can can be applied to a wide range of problems including: interface-surface comparisons, interface-interface comparisons and even comparisons of small molecule ligands. We demonstrate a use-case by applying InterComp to find similar protein interfaces on the surface of proteins. We show that InterComp pinpoints the correct interface for almost half of the targets (283 of 586) when considering the top 10 hits, and for 24% of the top 1, even when no templates can be found with the already available sequence-order dependent methods like TM-align.\n\nAvailabilityThe program is available from: http://wallnerlab.org/InterComp\n\nContactbjorn.wallner@liu.se\n\nSupplementary informationSupplementary data included in the pdf.