Noninvasive method for monitoring breathing patterns in monkeys

Abstract

Respiration is strongly linked to internal states such as arousal, emotion, and even cognitive processes and provides objective biological information to estimate these states in humans and animals. However, the measurement of respiration has not been established in macaque monkeys that have been widely used as model animals for understanding various higher brain functions. In the present study, we developed a method to monitor the respiration of behaving monkeys. We first measured the temperature of their nasal breathing, which changes between inspiration and expiration phases, in an anesthetized condition and estimated the respiration pattern. We compared the estimated pattern with that obtained by a conventional chest band method that has been used in humans and applies to anesthetized, but not behaving, monkeys. These respiration patterns matched well, suggesting that the measurement of nasal air temperature can be used to monitor the respiration of monkeys. Furthermore, we confirmed that the respiration frequency in behaving monkeys monitored by the measurement of nasal air temperature was not affected by the orofacial movement of licking to obtain the liquid reward. We next examined the frequency of respiration when they listened to music or white noise. The respiratory frequency was higher when the monkeys listened to music than the noise. This result is consistent with a phenomenon in humans and indicates the accuracy of our monitoring method. These data suggest that the measurement of nasal air temperature enables us to monitor the respiration of behaving monkeys and thereby estimate their internal states. Significance StatementWhile respiration is linked with internal processing, such as emotional and cognitive states, methods have not been established for physiological research on monkeys. We developed a novel method that obtained respiration signals by measuring the nasal air temperatures of behaving monkeys. Our method was able to continuously track the respiration pattern without distortions evoked by orofacial movements to lick the liquid reward. The respiratory frequency increased while listening to music than when listening to white noise for all monkeys. These results demonstrate that nasal air temperature measurements can be used to monitor the respiration patterns of aroused monkeys, allowing us to understand their internal state. This will be useful for investigating the underlying neuronal mechanism of neuropsychiatric disorders using monkeys.