The Dual Nature of Body-Axis Formation in Hydra Regeneration: Polarity-Morphology Concurrency

Abstract

The formation of a body-axis is central to animal development and involves both polarity and morphology. While polarity is traditionally associated with biochemical patterning, the morphological aspect of axis formation remains elusive. In regenerating Hydra tissues, we find that morphological evolution in all tissue samples depends on inherited positional information from the donors axis, and a foot precursor emerges early in the process. From the onset of regeneration, the Ca{superscript 2} excitations that drive actomyosin forces for tissue reshaping follow a gradient aligned with the head-foot polarity direction. We conclude that polarity and morphological axis progression occur concurrently through interlinked processes, and that the foot plays a dominant role in this process, a role usually attributed to the head organizer. A simple toy model accounts for the observed regeneration dynamics and illustrates the mechanochemical integration of polarity and morphogenesis. We expect the insights from Hydra to be relevant to broader developmental systems.