Effects of parity and early pregnancy on peripheral blood leukocytes in dairy cattle

Abstract

Subfertility remains a major problem in the dairy industry. Only 35-40% of high-yielding dairy cows and 55-65% of nonlactating heifers become pregnant after their first service. The immune system plays a critical role in the establishment of pregnancy. However, it can also create challenges for embryo survival and contribute to reduced fertility. We conducted 2 separate experiments to characterize changes in subsets of peripheral blood leukocytes (PBL) and their phenotype over the estrous cycle and early pregnancy in heifers and cows. We used flow cytometry and RT-qPCR to assess protein and mRNA expression of molecules important for immune function. We observed that monocytes and T cells were most affected by pregnancy status in heifers, whereas, CD8+ lymphocytes and natural killer (NK) cells were most affected during early pregnancy in cows. Changes in immune parameters measured appeared to be greater in heifers than cows including changes in expression of numerous immune function molecules. To test the hypothesis, we conducted a third experiment to simultaneously analyze the immunological responses to pregnancy between cows and heifers. We observed that cows had greater expression of proinflammatory cytokines and molecules associated with leukocyte migration and phagocytosis compared to heifers. Moreover, animals that failed to become pregnant showed altered expression of anti-inflammatory molecules. Overall, these findings support the hypothesis that early pregnancy signaling alters the proportions and functions of peripheral blood immune cells and differences between cows and heifers may yield insight into the reduced fertility of mature lactating dairy cows. Interpretative summaryIn dairy farming, 35-40% of high-yielding cows and 55-65% of nonlactating heifers conceive after their initial service. The immune system plays a crucial role in protecting cows from diseases and facilitating pregnancy establishment. This study investigates immune adaptation in bovine pregnancy by analyzing changes in immune cell populations and the expression of immunoregulatory molecules in the blood of heifers and lactating cows during early pregnancy. The findings support the hypothesis that early pregnancy signaling alters the proportions and functions of peripheral blood immune cells, offering insights into reduced fertility in mature lactating dairy cows.