Quantitative firing pattern phenotyping of hippocampal neuron types

Abstract

Systematically organizing the anatomical, molecular, and physiological properties of cortical neurons is important for understanding their computational functions. Hippocampome.org defines 122 neuron types in the rodent hippocampal formation (dentate gyrus, CA3, CA2, CA1, subiculum, and entorhinal cortex) based on their somatic, axonal, and dendritic locations, putative excitatory/inhibitory outputs, molecular marker expression, and biophysical properties such as time constant and input resistance. Here we augment the electrophysiological data of this knowledge base by collecting, quantifying, and analyzing the firing responses to depolarizing current injections for every hippocampal neuron type from available published experiments. We designed and implemented objective protocols to classify firing patterns based on both transient and steady-state activity. Specifically, we identified 5 transients (delay, adapting spiking, rapidly adapting spiking, transient stuttering, and transient slow-wave bursting) and 4 steady states (non-adapting spiking, persistent stuttering, persistent slow-wave bursting, and silence). By characterizing the set of all firing responses reported for hippocampal neurons, this automated classification approach revealed 9 unique families of firing pattern phenotypes while distinguishing potential new neuronal subtypes. Several novel statistical associations also emerged between firing responses and other electrophysiological properties, morphological features, and molecular marker expression. The firing pattern parameters, complete experimental conditions (including solution and stimulus details), digitized spike times, exact reference to the original empirical evidence, and analysis scripts are released open-source through Hippocampome.org for all neuron types, greatly enhancing the existing search and browse capabilities. This information, collated online in human-and machine-accessible form, will help design and interpret both experiments and hippocampal model simulations.\n\nSignificance StatementComprehensive characterization of nerve cells is essential for understanding signal processing in biological neuronal networks. Firing patterns are important identification characteristics of neurons and play crucial roles in information coding in neural systems. Building upon the comprehensive knowledge base Hippocampome.org, we developed and implemented automated protocols to classify all known firing responses exhibited by each neuron type of the rodent hippocampus based on analysis of transient and steady-state activity. This approach identified the most distinguishing elements of every firing phenotype and revealed previously unnoticed statistical associations of firing responses with other electrophysiological, morphological, and molecular properties. The resulting data, freely released online, constitute a powerful resource for designing and interpreting experiments as well as developing and testing hippocampal models.