bioRxivBiophysicsPreprint
Focused acoustic-radiation-force microscopy for live-cell selective nucleus deformation and nucleus viscoelasticity measurement
Natsumi Fujiwara, Hiroki OkitaiD, Midori Uno, Kanta AdachiiD, Kichitaro NakajimaiD, Nobutomo NakamuraiD, Mee‐Hae KimiD, Masahiro Kino‐okaiD, Kotaro OgawaiD, Kensuke IkenakaiD, Hirotsugu OgiiD
Abstract
Acoustic-radiation-force (ARF) microscopy was developed for non-invasive, selective mechanical stimulation to a single live-cell nucleus and long-term measurement of its mechanical properties. High-frequency focused ultrasound wave achieved a spatially confined ARF field around target nucleus, and its cyclic deformation was induced by amplitude modulation on the ultrasound wave, which was captured by a dark-field microscopy. This method was applied to human mesenchymal stem cells, revealing their extremely high mechanical homeostasis.
- organism: human
- assay: imaging
- research focus: cell signaling & trafficking
- approach: microscopy & imaging analysis
- Citations
- 0
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